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—vŽ|F•ªŽqƒ}[ƒJ[‚ð—˜—p‚µ‚Ä—L—pˆâ“`Žq‚ðŒø—¦“I‚É“±“ü‚·‚éˆçŽí–@‚ðŠm—§‚·‚é–Ú“I‚ŁCˆâ“`‰ðÍ‚̍ޗ¿‚Æ‚µ‚āC“¿“‡ƒ‚ƒ`—‡—R—ˆ‚̃IƒIƒ€ƒMŽÈˆÞk•a’ïR«•iŽíŽÈŒn6‚ƃIƒIƒ€ƒMŽÈˆÞk•aœë•a«•iŽílk2‚ÌF1‚ɖ쐶ƒIƒIƒ€ƒMcb2920‚ðŒð”z‚µ‚Ä“¾‚½ãó‚ð”|—{‚µCƒRƒ‹ƒqƒ`ƒ“ˆ—‚ð‚µ‚Ä”¼”‘Ì”{‰ÁŒn“‚ðìo‚µ‚½D”¼”‘Ì”{‰ÁŒn“ŒQ‚̍ìo—¦‚́C–쐶ƒIƒIƒ€ƒM‚ðŽó•²‚µ‚½F1‚̉o‰Ô”‚ɑ΂µ‚āC1.7`4.5“‚Å‚ ‚Á‚½D”¼”‘Ì”{‰ÁŒn“ŒQ95Œn“‚̃IƒIƒ€ƒMŽÈˆÞk•a (‡TŒ^) ‚ɑ΂·‚é•\Œ»Œ^‚́C’ïR«47Œn“‚ÆŠ´Žó«48Œn“‚ŁCŠú‘Ò•ª—£”ä1F1‚É‚æ‚­ˆê’v‚µ‚½D‚Ü‚½C˜A½•ªÍ‚É—˜—p‚·‚邽‚ß‚Ì•ªŽqƒ}[ƒJ[‚É‚æ‚茟o‚µ‚½ˆâ“`ŽqŒ^‚Ì•ª—£”ä‚àC26ƒ}[ƒJ[’†25ƒ}[ƒJ[‚ªŽÈŒn6Œ^Flk2Œ^‚ª1F1‚ÌŠú‘Ò•ª—£”ä‚É“K‡‚µCƒwƒeƒŒ^‚Í‘S‚­ŒŸo‚³‚ê‚È‚©‚Á‚½D”¼”‘Ì”{‰ÁŒn“ŒQ‚͈â“`‰ðÍ‚̍ޗ¿‚Æ‚µ‚āCŠ®‘S‚ȃzƒ‚Ú‡‘̂ŃwƒeƒŒ^‚ª”»’è‚Å‚«‚È‚¢—D«ƒ}[ƒJ[‚à—LŒø‚ÉŽg‚¦‚邱‚ƁC•\Œ»Œ^‚¨‚æ‚шâ“`ŽqŒ^‚Ì•ª—£”䂪’Pƒ‚Å‚ ‚邱‚ƁC“¯ˆê‚̈â“`Žq\¬‚̍ޗ¿‚Æ‚µ‚ĈێE‘B‚ª—eˆÕ‚È‚½‚߁CˆÙ‚È‚éŠÂ‹«‰º‚ÅŒ`Ž¿•]‰¿‚ðŒJ‚è•Ô‚µs‚¤‚±‚Æ‚ª‰Â”\‚ŁCˆâ“`Žq”­Œ»‚Ì•]‰¿‚̐M—Š«‚ð‚‚ß‚é‚±‚Æ‚ª‚Å‚«‚é“_‚Å—D‚ê‚Ä‚¢‚½D

ƒL[ƒ[ƒhF˜A½‰ðÍC”¼”‘Ì”{‰ÁŒn“Cƒr[ƒ‹‘唞C•ªŽqƒ}[ƒJ[CH.bulbosum L.
Production of Doubled Haploid Lines for Genetis Analysis in Useful Characters of Malting Barley: Yosuke UCHIMURA, Masahiko FURUSHO, Takahide Baba, Osamu Yamaguchi, Hiroomi Kai, Morihiro Tsukazaki and Tomohiko Yoshida
Abstract: To Develop a DNA marker assisted selection system for lines with recessive resistance gene to all races of barley yellow mosaic virus (BaYMV), we produced doubled haploid lines by the bulbosum method in barley (Hordeum vulgare L.). We used Japanese six-rowed barley cultivar Shimakei6 with a resistance gene to all races of BaYMV and susceptible two-rowed barley cultivar lk2. Double haploid lined were obtained by pollinating the F1 plants with pollen of H.bulbosum L.. Ratio of haploid plants obtained to florets pollinated was 1.7 - 4.5 %. Ninety five haploid lines segregated to 47 resistance and 48 susceptible to BaYMV strain I in the infected field by BaYMV. This segregation ratio fitted a theoretical ratio of 1:1. Segregation ratios by molecular markers also fitted a theoretical ratio of 1:1 except one marker. No heterozygous genotype was observed. Double haploid lines are useful because we could obtained fixed lines in a short piriod and doubled haploid lines have complete homogenote phenotype even with recessive genotype. Reliable evaluation of phenotype by many times is possible because they are fixed without segregation by seed multiplication.
Key words; Barley, Doubled haploid lines, Genetic analysis, H. bulbosum L., Molecular marker.

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Blake, T.K, D. Kadqrzhanova, K.W. Shepherd, A.K.M.R. Islam, P.L. Langridge, C.L. McDonald, 
@J.Erpelding, S.Larson, N.K. Blake and L.E.Talbert 1996DSTS-PCR markers appropriate for
    wheat-barley introgressionDTheor. Appl. Genet. 93: 826|832D
•Ÿ‰ª’‰•FEŒÃ¯‰ë•FE–q–ì“¿•F 1991.@ƒ‚ƒ`«ƒIƒIƒ€ƒM•iŽíe“¿“‡ƒ‚ƒ`—‡f‚É‘¶Ý‚·‚éV‚µ‚¢ŽÈˆÞk•a’ïR«ˆâ“`Žq‚ɂ‚¢‚Ä.
@ˆçŽG41 (•Ê2):388\389D 
ŒÃ¯‰ë•FE•l’n—EŽŸEˆÉ“¡¹Œõ 1987.@”¼”‘Ì—˜—p‚É‚æ‚éƒr[ƒ‹‘唞‚̈çŽí•û–@D‚PDŒø—¦“I‚È”¼”‘̂̍ìo–@.
@“úì‹ãŽx•ñ 54:92\94D@ 
ŒÃ¯‰ë•FE•l’n—EŽŸE‹g“c’q•F 1988.@ƒIƒIƒ€ƒM”¼”‘Ì”{‰ÁŒn“‚Ì‘å—ʍìo–@‚Æ”¼”‘Ì‚É‚¨‚¯‚邤‚Ç‚ñ‚±•a’ïR«‚Ì‘I”².
@ˆçŽG38 (•Êû2):336\337D
ŒÃ¯‰ë•FE•l’n—EŽŸE‹g“c’q•FE’†“‡ŽH‰î 1990.@”¼”‘Ì‚É‚¨‚¯‚éƒIƒIƒ€ƒMŽÈˆÞk•a‚Ì‘I”².@ˆçŽG40 (•Êû1):290\291D
FURUSHO,M., Y.HAMACHI and T.YOSHIDA 1990. Varietal difference in cross-ability between Japanese
@ two-lowed barley and Hordeum bulbosum L. Japan. J. Breed. 40:411\417. 
ŒÃ¯‰ë•FE‹g“c’q•F 1992.@Bulbosum–@‚É‚æ‚蓾‚ç‚ꂽƒr[ƒ‹‘唞‚Ì”¼”‘Ì”{‰ÁŒn“‚Ì”_‹ÆŒ`Ž¿‚Æ”ž‰è•iŽ¿.
@ˆçŽG 42:631\639D
ŒÃ¯‰ë•FE•Ÿ‰ª’‰•F 1997.@ƒIƒIƒ€ƒMŽÈˆÞk•a’ïR«‚̈â“`Žq•ªÍ‚Æ‚»‚Ì—˜—pD”_Œ¤ƒZƒ“ƒ^[Œ¤•ñ27:31\71D
Furusho,M., T.Baba, O.Yamaguchi, T.Yoshida, Y.Hamachi, R.Yoshikawa, K.Mizuta and M. Yoshino 1999. 
  Breeding of a new malting barley cultivar Hoshun by the Bulbosum method Japan. J. Breed. 49:281\284.
Kai,H., T.Baba, M.Tsukazaki, Y.Uchimura and M.Furusho 2003. The QTL analysis of hull-cracked
   grain in Japanese malting barley. Breeding Sci. 53:225\230. 
Konieczny,A. and F.M.Ausubel 1993. A procedure for mapping Arabidopsis mutations using co-dominant
@ ecotype-specific PCR-based markers. Plant J. 4: 403\410.  
Kubo,T., Y.Aida, K.Nakamura, H.Tsunematsu, K.Doi and A.Yoshimura 2002. Reciprocal chromosome segment
@ substitution series derived from japonica and indica cross of rice (Oryza sativa L.). Breeding Sci.
   52:319\325. 
‘ê•q•FE‰“ŽR–¾EŒš•”ŽÀŽŸ 1965.@‘唞ŽÈˆÞk•a‚ɑ΂·‚éƒr[ƒ‹”ž•iŽí‚Ì”íŠQ’ïR‚ɂ‚¢‚Ä.
@’†‘”_‹ÆŒ¤‹† 33:36\38D
Miyazaki,C., E.Osanai, K.Saeki, K.Ito, T.Konishi, K.Sato and A.Saito 2001. Mapping of quantitative
  trait loci conferring resistance to barley yellow mosaic virus in a Chinese
  barley landrace Mokusekko 3. Breeding Sci. 51:171\177. 
‰ª“c‹gOE–ØŒ´½EÄ“¡ÂE‰Í“c®”VEˆÉ“¡ˆê•q 2002.@“ú–{‚¨‚æ‚Ñ–k•Ä‚Ì—D—ǃr[ƒ‹‘唞•iŽí‚ð—p
  ‚¢‚½”ž‰è•iŽ¿‚̃Qƒmƒ€‰ðÍ.@ˆçŽíŠwŒ¤‹†4:320D
‚‹´‹`s 1988.@ELISA–@\‚»‚Ì“Á’¥‚ÆŽÀŽ{ã‚Ì’ˆÓ“_\.@A•¨–h‰u 42:88\92D
‰“ŽR–¾E‘—t•q•F 1970.@ƒIƒIƒ€ƒMŽÈˆÞk•a‚Ì“`õ‚ɂ‚¢‚Ä:‘æ2•ñ Polymyxa@graminis Led. ‚É‚æ‚é”}‰î.
@“úA•a•ñ 36:223\229D
ŽŒ´˜alE“¡ˆä•q’jE–ì‘ò´ˆêEŠÖŒû’‰’j 1984.@‘唞ŽÈˆÞk•a‚ƃr[ƒ‹‘唞•iŽ¿.@ˆçŽG 34:302\303D
–î–쏹—TEt“‡‰ÃÍ 1994.@•ªŽqƒ}[ƒJ[‚ð—˜—p‚µ‚½ƒCƒl—Ê“IŒ`Ž¿‚̈â“`‰ðÍ.@ˆçŽíŠwÅ‹ß‚̐i•à 36:29\32D
‹g‘º~E“yˆäˆês 2001. ƒOƒ‰ƒxƒŠƒ}ˆî‚̈â“`“Á«‚̉ðÍ|õF‘Ì’f•Ð“±“üŒn“@‚̍쐬‚Æ—˜—p|.@
  ‘Û”_‹ÆŒ¤‹†î•ñ22:15\21D 



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                                            „ DNA‘½Œ^  ”¼”‘Ì”{‰ÁŒn“ŒQ          
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                 „Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ@„ @@     @‚̈â“`ŽqŒ^•ª—£”ä        
@@@@•ªÍ•û–@                            „  ŒŸo—¦                            
                     ’²¸”     DNA‘½Œ^    „ @@@@@ ‚ª1F1‚É“K‡‚µ‚½        
                                           „                                    
                       (a)      ŒŸo”(b)  „  (b/a%)@  •ªŽqƒ}[ƒJ[” ‚Pj     
    „Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ@    
       @SSR       „ @ 59          13          22.0    „   12                  
         CAPS      „    47          17          36.2    „   17                  
         RAPD‚Qj   „  4008           7           0.2    „    7                  
    „Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„¨„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ„Ÿ@    
         ‚PjŽÈŒn6‚Ælk2Œð”zF1—R—ˆ‚Ì94‚Ì”¼”‘Ì”{‰ÁŒn“‚𒲍¸‚µC         
             ƒÔ2ŒŸ’è‚ð—LˆÓ…€5“‚ōs‚Á‚½Œ‹‰ÊD                           
         ‚Qjƒoƒ‹ƒN•ªÍ‚Å‚ÌŒ‹‰ÊD
            
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